Multiple headspace solid-phase microextraction for quantifying volatiles in <italic style="font-style: italic">Citrus reticulata</italic> 'Chachi' peel of different storage time

HU Shuangfei; ZHENG Yuying; XIE Shiting; SU Weiwei; PENG Wei; LI Peibo; WU Hao

doi:10.13471/j.cnki.acta.snus.ZR20240303

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Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time

Column on Modernization of Traditional Chinese Medicine（Contributing editor： SU Weiwei） | 更新时间：2026-05-22

- Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time
- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 65, Issue 3, Pages: 33-42(2026)
- 作者机构：
  
  广东省中药上市后质量与药效再评价工程技术研究中心 / 广东省植物逆境生物学重点实验室 / 中山大学生命科学学院, 广东广州 510275
- 作者简介：
  
  HU Shuangfei（hushf8@mail2.sysu.edu.cn）
  ZHENG Yuying（vicky_0224@126.com）
  XIE Shiting（xieshiting@163.com）
  SU Weiwei（lsssww@126.com）
  PENG Wei（pengwei3@mail.sysu.edu.cn）
  LI Peibo（lipeibo@mail.sysu.edu.cn）
  WU Hao（wuhao8@mail.sysu.edu.cn）
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.ZR20240303
  CLC： Q94
- Received：15 October 2024，
  
  Revised：2024-12-12，
  
  Accepted：14 January 2025，
  
  Online First：26 March 2025，
  
  Published：25 May 2026
- 稿件说明：
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胡双飞,郑玉莹,谢诗婷等.多次顶空固相微萃取在不同年份广陈皮挥发性化合物定量中的应用[J].中山大学学报(自然科学版)(中英文),2026,65(03):33-42.

HU Shuangfei,ZHENG Yuying,XIE Shiting,et al.Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2026,65(03):33-42.
胡双飞,郑玉莹,谢诗婷等.多次顶空固相微萃取在不同年份广陈皮挥发性化合物定量中的应用[J].中山大学学报(自然科学版)(中英文),2026,65(03):33-42. DOI： 10.13471/j.cnki.acta.snus.ZR20240303.

HU Shuangfei,ZHENG Yuying,XIE Shiting,et al.Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2026,65(03):33-42. DOI： 10.13471/j.cnki.acta.snus.ZR20240303.

摘要

采用多次顶空固相微萃取（MHS-SPME，multiple headspace solid-phase microextraction）结合气相色谱-质谱联用（GC-MS，gas chromatography-mass spectrometry）技术，定量分析了不同年份广陈皮中的挥发性成分。通过考察萃取时间、萃取温度、样品量以及不同萃取头涂层，建立了采用多次顶空固相微萃取（MHS-SPME）对广陈皮中D-柠檬烯、

γ-

萜品烯、L

α-萜品醇和2-甲氨基苯甲酸甲酯含量测定方法。定性出50种挥发性化合物，包括萜类、醇类、醛类和酯类。结果显示，广陈皮中的萜类化合物随着陈化年份增加含量下降快于酯类和醇类。该发现有助于揭示广陈皮在陈化过程中挥发性化合物的变化。

Abstract

The dried pericarp of

Citrus reticulata

'Chachi' (GCP

"Guangchenpi" in Chinese) is widely used in traditional Chinese medicine and cuisine. It has been generally recognized that its health benefits are dependent on storage time. As storage years increase

the most relevant components that are volatile oils. However

there are very few reports on the change in the content of volatiles in GCP with the increase of storage years. In this study

we examined the volatiles of GCP based on the headspace solid-phase microextraction method and the gas chromatography-mass spectrometry (HS-SPME-GC-MS). Factors of the extraction duration and temperature

sample size

and the type of fiber coatings were evaluated to quantify the D

Limonene

γ-Terpinene

L-α

Terpineol

and 2-(Methylamino)-benzoic acid-methyl ester in the GCP using the multiple headspace solid-phase microextraction approach (MHS-SPME). A total of 50 volatile compounds were identified

including terpenic

alcohol

aldehyde

and ester series. Results show that the terpenic series in the GCP decays much quicker than

the ester and alcohol. The findings in the study shall help discriminate the behavior of the volatile substance during the GCP aging.

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references

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Related Author

HU Shuangfei

ZHENG Yuying

XIE Shiting

SU Weiwei

PENG Wei

LI Peibo

WU Hao

PENG Wei

Related Institution

School of Life Sciences, Sun Yat-sen University / Guangdong Engineering and Technology Research Center for Ouality and Efficacy Re-valuation of Post-marketed TCM / Guangdong Provincial Key Laboratory of Plant Stress Biology

Department of Chemical Engineering， Maoming Polytechnic

Maoming Branch， Guangdong Laboratory for Lingnan Modern Agriculture

School of Life Sciences， Sun Yat-sen University / Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-valuation of Post-marketed TCM / Guangdong Key Laboratory of Plant Resources

．中山大学药学院生药学与天然药物化学实验室

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Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time

DOI：10.13471/j.cnki.acta.snus.ZR20240303

摘要

Abstract

关键词

Keywords

references