Shi Huahong, Yang Yansheng, Chen Panzao, et al. A Time-resolved Fluoroimmunoassay for Determination of Human Immunoglobulin (IgG) in Urine-Streptavidin-Eu (Ⅲ) as a label in immunoassay[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1996,35(2):71-74.
Shi Huahong, Yang Yansheng, Chen Panzao, et al. A Time-resolved Fluoroimmunoassay for Determination of Human Immunoglobulin (IgG) in Urine-Streptavidin-Eu (Ⅲ) as a label in immunoassay[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1996,35(2):71-74.DOI:
Sheep anti human IgG antibody was immobilized by adsorption to the walls of polystyrens microtiter strips. Human IgG was biotinylated. Biotinylated human IgG and sample human IgG competed for the limited antibody on solid phase. After the immunoreaction was completed
biotin on solid phase can "catch" SA-Eu (Ⅲ) Eu (Ⅲ) fluorescent counts was measured on a time-resolved fluorometer with addition fluorescent enhancement solution. The range of the assay standard curve is 0. 625 ̄ 40. 0 mg/L.This method can be used to determine content of igG in urine. In addition