广东省名特优鱼类生殖调控与繁育工程技术研究中心 / 广东省水产经济动物病原生物学及流行病学重点实验室 / 广东省海水养殖生物育种工程实验室 / 广东海洋大学水产学院,广东 湛江 524088
黄春艳(2000年生),女;研究方向:鱼类生理;E-mail:2112201146@stu.gdou.edu.cn
陈华谱(1983年生),男;研究方向:鱼类生殖生长及性别调控;E-mail:chenhp@gdou.edu.cn
纸质出版日期:2025-01-15,
网络出版日期:2024-10-22,
收稿日期:2024-07-07,
录用日期:2024-08-12
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黄春艳,陈华谱,郭煜文等.卵形鲳鲹促性腺激素β亚基的克隆鉴定及其受雌二醇的调控[J].中山大学学报(自然科学版)(中英文),2025,64(01):172-181.
HUANG Chunyan,CHEN Huapu,GUO Yuwen,et al.Cloning and characterization of gonadotropin β subunit genes in pompano (Trachinotus ovatus) and their regulation by estradiol[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2025,64(01):172-181.
黄春艳,陈华谱,郭煜文等.卵形鲳鲹促性腺激素β亚基的克隆鉴定及其受雌二醇的调控[J].中山大学学报(自然科学版)(中英文),2025,64(01):172-181. DOI: 10.13471/j.cnki.acta.snus.ZR20240224.
HUANG Chunyan,CHEN Huapu,GUO Yuwen,et al.Cloning and characterization of gonadotropin β subunit genes in pompano (Trachinotus ovatus) and their regulation by estradiol[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2025,64(01):172-181. DOI: 10.13471/j.cnki.acta.snus.ZR20240224.
促性腺激素(GtH,gonadotropin hormone)是垂体合成和分泌的一类重要糖蛋白激素,包括促卵泡
生成素(Fsh,follicle stimulating hormone)和促黄体生成素(Lh,luteinizing hormone),在促进性腺发育和成熟过程中发挥重要作用。本研究成功克隆了卵形鲳鲹(
Trachinotus ovatus
)
fshβ
和
lhβ
基因的开放阅读框(ORF,open reading frame)序列,
fshβ
ORF长度为363 bp,
lhβ
ORF长度为447 bp。通过荧光定量PCR技术(qPCR,quantitative real-time PCR)检测,发现
fshβ
和
lhβ
基因在卵形鲳鲹的下丘脑-垂体-性腺轴(HPG,hypothalamus-pituitary-gonadal axis)显著表达,在垂体中的表达水平最高,其次是下丘脑和性腺。此外,通过体外实验评估17β-雌二醇(E2,17β-estradiol)对卵形鲳鲹垂体组织中
fshβ
和
lhβ
基因表达的影响。结果显示,10 μmol/L E2处理6 h后,GtH β亚基基因的表达被极显著抑制。最后,使用雌激素受体(ER,estrogen receptor)拮抗剂体外孵育卵形鲳鲹垂体组织,发现广谱性拮抗剂Fulvestrant、ERβ拮抗剂Cyclofenil和ERα拮抗剂MPP(MPP dihydrochloride)均能够消除E2对GtH β亚基基因表达的抑制作用。综上所述,研究结果表明E2对卵形鲳鲹GtH的分泌具有负反馈调节作用,为卵形鲳鲹生殖调控机制提供了有价值的见解。
Gonadotropin hormones (GtH) are a crucial class of glycoprotein hormones synthesized and secreted by the pituitary, playing a pivotal role in promoting gonadal development and maturation. In this study, we successfully cloned the open reading frame(ORF) sequences of
fshβ
and
lhβ
from pompano
(
Trachinotus ovatus
), with
fshβ
ORF length of 363 bp and
lhβ
ORF length of 447 bp. Using quantitative real-time PCR(qPCR), we observed expression of
fshβ
and
lhβ
in various tissues of pompano, with the highest levels detected in the pituitary followed by the hypothalamus. Furthermore,
in vitro
experiments were conducted to assess the effect of 17β-estradiol(E2) on
fshβ
and
lhβ
expression in pompano pituitary. Results showed a significant inhibition of
fshβ
and
lhβ
expression after 6 h of treatment with 10 μmol/L E2. Lastly, using estrogen receptor(ER) antagonists for
in vitro
incubation of pompano pituitary tissue, we demonstrated that bro
ad-spectrum antagonist Fulvestrant, ERβ antagonist Cyclofenil, and ERα antagonist MPP dihydrochloride could alleviate the inhibitory effect of E2
on
fshβ
and
lhβ
expression. Overall, our findings suggest a negative feedback regulation of E2 on GtH secretion in pompano, providing valuable insights into the reproductive regulation mechanism of pompano.
卵形鲳鲹(Trachinotus ovatus)促性腺激素(GtH)雌激素受体(ER)17β-雌二醇(E2)
pompano(Trachinotus ovatus)gonadotropin hormones (GtH)estrogen receptor(ER)17β-estradiol(E2)
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