Shi Suhua, Zhang Qun, Chen Yueqin, et al. A Simple Method for Isolation of Total RNA and DNA from Silicagel-dried and Fresh Leaves of Plants[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1996,35(2):103-109.
Shi Suhua, Zhang Qun, Chen Yueqin, et al. A Simple Method for Isolation of Total RNA and DNA from Silicagel-dried and Fresh Leaves of Plants[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1996,35(2):103-109.DOI:
A simple and rapid method was proposed for the isolation of total DNA and RNA from silicageldryed leaves as well as fresh leaves of various groups of plants. without special clearing treatments of solutions
glassware and plasticware
and the use of poisonous phenol. This method is fairely suitable for the isolation of DNA and RNA from collection of plant leaves dryed in silicagel in field Which is convenient for international exchange. The isolated total DNA and RNA can be applied effectively in PCR
sequencing. SnRNA studies and other molecular biological researches.
关键词
&nbsp植物核酸提取硅胶干燥保存RNA和DNA
Keywords
&nbspefficient methodisolationtotal DNA and RNA