芸苔属几种蔬菜种子的劣变及其荧光泄漏物质Ⅱ．荧光泄漏物质的监定和种子活力与泄漏液芥子碱含量的相关性

黄学林; 李燕红; 傅家瑞

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芸苔属几种蔬菜种子的劣变及其荧光泄漏物质Ⅱ．荧光泄漏物质的监定和种子活力与泄漏液芥子碱含量的相关性

论文 | 更新时间：2023-12-11

- 芸苔属几种蔬菜种子的劣变及其荧光泄漏物质Ⅱ．荧光泄漏物质的监定和种子活力与泄漏液芥子碱含量的相关性
- Seed Deterioration in Several Brassica Vagetative Species and Their Fluorescent LeakageⅡ. Identification of the fluorescent leak age by paper chromatography and the seed vigor test by sinapine leakage
- 中山大学学报(自然科学版)(中英文) 1995年34卷第1期页码：126-129
- 作者机构：
  
  中山大学生物学系
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 纸质出版日期：1995，
  
  网络出版日期：1995-1-25，
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黄学林, 李燕红, 傅家瑞. 芸苔属几种蔬菜种子的劣变及其荧光泄漏物质Ⅱ．荧光泄漏物质的监定和种子活力与泄漏液芥子碱含量的相关性[J]. 中山大学学报(自然科学版)(中英文), 1995,34(1):126-129.

Huang Xuelin, Li Yanhong, Fu Jiarui. Seed Deterioration in Several Brassica Vagetative Species and Their Fluorescent LeakageⅡ. Identification of the fluorescent leak age by paper chromatography and the seed vigor test by sinapine leakage[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1995,34(1):126-129.
黄学林, 李燕红, 傅家瑞. 芸苔属几种蔬菜种子的劣变及其荧光泄漏物质Ⅱ．荧光泄漏物质的监定和种子活力与泄漏液芥子碱含量的相关性[J]. 中山大学学报(自然科学版)(中英文), 1995,34(1):126-129. DOI：

Huang Xuelin, Li Yanhong, Fu Jiarui. Seed Deterioration in Several Brassica Vagetative Species and Their Fluorescent LeakageⅡ. Identification of the fluorescent leak age by paper chromatography and the seed vigor test by sinapine leakage[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1995,34(1):126-129. DOI：

摘要

用纸层析结合显色反应初步鉴定了芥蓝，白菜，菜心劣变种子中的荧光泄漏物质，发现它们均含有Ｒｆ值、显色反应与芥子碱相同的化合物，根据芥子碱在ｐＨ＞１０时转变为黄色，最大吸收波长为３８８ｎｍ的特征，利用光密度测定法检验该类种子活力，并计算两者之间的相关系数，其ｒ分别为－０．９２，－０．８１和－０．８１，实验表明，用光密度（３８８ｎｍ）测定法检测这些种子活力是可行的，并且比荧光观测法灵敏，快速，准确。

Abstract

The fluorescent leakage from Brassicu alboglabra Bailey

B. chinensis Linn

and B.parachinensis bailey was identified by paper chromatography.The leakage was found to contain acampound

the R_f value and color reaction of which were similar to that of sinapine

The concen-tration of the sinapine determinated by using the specific absorbance of the compound at 388nmin basic solution(pH> 1 0)was closely associated with the seed vigor.Their relative coefficiencywere respectively-0. 92

-0. 81 and -0. 81.Thus

the seed vigor test in these species utilizingsinapine determination is practical

sensitive and better than method of observation of the fluores-cent leakage under UV light.

关键词

芸苔属种子芥子碱活力测定

Keywords

&nbspBrassica seed fluorescent leakageseed vigor test

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