Wang Yiliang, Zhang Tianyuan, Luo Jinxian. Selection of Pullulanase Producing Strain A 3345[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1996,35(S2):54-57.
Wang Yiliang, Zhang Tianyuan, Luo Jinxian. Selection of Pullulanase Producing Strain A 3345[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1996,35(S2):54-57.DOI:
A Aerobacter aerogenes mutant producing high pullulanas eactivity was selected by reciprocal or mixed treatment of the parental strain with NTG; U.V and HNO 2. The pullulanase activity of the selected strain is 750 u/mL in Teng Wei (D M) medium (A) after 4 days incobation at 30℃
which is 110% higher than the parental strain. The optimal pH and temperature is 5 2~6 4 and 50℃ respectively.