Deletion of Cloned DNA Fragment in M13 and Application of Bluescript Vector for Subcloning and Sequencing[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1988,27(2).
Deletion of Cloned DNA Fragment in M13 and Application of Bluescript Vector for Subcloning and Sequencing[J]. Acta Scientiarum Naturalium Universitatis SunYatseni, 1988,27(2).DOI:
A Dictyostelium DNA fragment cloned into Ml3 for sequencing deleted during subcloning. The Bluescript M13 vector and exonuclease-Ⅲ were employed to produce a sequential series of overlapping clones for sequencing. The sequencing procedure was simplified by using supercoiled double strand DNA as template.