Multiple headspace solid-phase microextraction for quantifying volatiles in <italic style="font-style: italic">Citrus reticulata</italic> 'Chachi' peel of different storage time

HU Shuangfei; ZHENG Yuying; XIE Shiting; SU Weiwei; PENG Wei; LI Peibo; WU Hao

doi:10.13471/j.cnki.acta.snus.ZR20240303

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Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time

更新时间：2025-03-24

- Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time
- Acta Scientiarum Naturalium Universitatis Sunyatseni Pages: 1-10(2025)
- 作者机构：
  
  中山大学生命科学学院 / 广东省中药上市后质量与药效再评价工程技术研究中心 / 广东省植物逆境生物学重点实验室, 广东广州 510275
- 作者简介：
  
  HU Shuangfei（hushf8@mail2.sysu.edu.cn）；
  ZHENG Yuying（vicky_0224@126.com）；
  XIE Shiting（xieshiting@163.com）；
  SU Weiwei（lsssww@126.com）；
  PENG Wei（pengwei3@mail.sysu.edu.cn）；
  LI Peibo（lipeibo@mail.sysu.edu.cn）
  WU Hao（wuhao8@mail.sysu.edu.cn）
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.ZR20240303
  CLC： Q94
- Received：15 October 2024，
  
  Accepted：2025-01-14，
  
  Published Online：2025-03，
- 稿件说明：
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HU Shuangfei, ZHENG Yuying, XIE Shiting, et al. Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time[J/OL]. Acta scientiarum naturalium universitatis sunyatseni, 2025, 1-10.
DOI：

HU Shuangfei, ZHENG Yuying, XIE Shiting, et al. Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time[J/OL]. Acta scientiarum naturalium universitatis sunyatseni, 2025, 1-10. DOI： 10.13471/j.cnki.acta.snus.ZR20240303.

摘要

采用多次顶空固相微萃取（MHS-SPME，multiple headspace solid-phase microextraction）结合气相色谱-质谱联用（GC-MS，gas chromatography-mass spectrometry）技术，定量分析了不同年份广陈皮中的挥发性成分。通过考察萃取时间、萃

取温度、样品量以及不同萃取头涂层，建立了采用多次顶空固相微萃取（MHS-SPME）对广陈皮中

-柠檬烯、

-萜品烯、

L-α

-萜品醇和2-甲氨基苯甲酸甲酯含量测定方法。研究共定性出挥发性化合物50种，包括萜类、醇类、醛类和酯类。结果显示，广陈皮中的萜类化合物随着陈化年份增加含量下降快于酯类和醇类。该发现有助于揭示广陈皮在陈化过程中挥发性化合物的变化。

Abstract

The dried pericarp of

Citrus reticulata

'Chachi' (GCP

"Guangchenpi" in Chinese) is widely used in traditional Chinese medicine and cuisine. It has been generally recognized that its health benefits are dependent on storage time. As storage years increase

the most relevant components that are volatile oils. However

there are very few reports on the change in the content of volatiles in GCP with the increase of storage years. In this study

we examined the volatiles of GCP based on the headspace solid-phase microextraction method and the gas chromatography-mass spectrometry (HS-SPME-GC-MS). Factors of the extraction duration and temperature

sample size

and the type of fiber coatings were evaluated to quantify the

D-

Limonene

-Terpinene

L-α-

Terpineol

and 2-(Methylamino)-benzoic acid-methyl ester in the GCP using the multiple headspace solid-phase microextraction approach (MHS-SPME). A total of 50 volatile compounds were identified

including terpenic

alcohol

aldehyde

and ester series. Results show that the terpenic series in the GCP decays much quicker than the ester and alcohol. The findings in the study shall help discriminate the behavior of the volatile substance during the GCP aging.

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references

BIAN X ， XIE X ， CAI J ， et al ， 2022 . Dynamic changes of phenolic acids and antioxidant activity of Citri Reticulatae Pericarpium during aging processes ［J］. Food Chem ， 373 ： 131399 .

COSTA R ， TEDONE L ， de GRAZIA S ， et al ， 2013 . Multiple headspace-solid-phase microextraction： An application to quantification of mushroom volatiles ［J］. Anal Chim Acta ， 770 ： 1 - 6 .

DUETZ W A ， FJÄLLMAN A H ， REN S ， et al ， 2001 . Biotransformation of D-limonene to （+） trans-carveol by toluene-grown Rhodococcus opacus PWD4 cells ［J］. Appl Environ Microbiol ， 67 （ 6 ）： 2829 - 2832 .

FU M ， XU Y ， CHEN Y ， et al ， 2017 . Evaluation of bioactive flavonoids and antioxidant activity in Pericarpium Citri Reticulatae （ Citrus reticulata ‘Chachi’） during storage ［J］. Food Chem ， 230 ： 649 - 656 .

GORINSTEIN S ， MARTı́N-BELLOSO O ， PARK Y S ， et al ， 2001 . Comparison of some biochemical characteristics of different Citrus fruits ［J］. Food Chem ， 74 （ 3 ）： 309 - 315 .

HE J ， CHEN C ， HE Q ， et al ， 2019 . The central bacterial community in Pericarpium Citri Reticulatae ‘Chachiensis’ ［J］. Food Res Int ， 125 ： 108624 .

KASHIWAGI T ， LAN PHI N T ， SAWAMURA M ， 2010 . Compositional changes in Yuzu （ Citrus junos ） steam-distilled oil and effects of antioxidants on oil quality during storage ［J］. Food Sci Technol Res ， 16 （ 1 ）： 51 - 58 .

LEI F F ， ZHANG X N ， GAO Y L ， et al ， 2012 . Multiple headspace solid-phase microextraction using a new fiber for avoiding matrix interferences in the quantitative determination of ethyl carbamate in pickles ［J］. J Sep Sci ， 35 （ 9 ）： 1152 - 1159 .

LIU E H ， ZHAO P ， DUAN L ， et al ， 2013 . Simultaneous determination of six bioactive flavonoids in Citri Reticulatae Pericarpium by rapid resolution liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry ［J］. Food Chem ， 141 （ 4 ）： 3977 - 3983 .

MINH TU N T ， THANH L X ， UNE A ， et al ， 2002 . Volatile constituents of Vietnamese pummelo， orange， tangerine and lime peel oils ［J］. Flavour & Fragrance J ， 17 （ 3 ）： 169 - 174 .

NGUYEN H ， CAMPI E M ， ROY JACKSON W ， et al ， 2009 . Effect of oxidative deterioration on flavour and aroma components of lemon oil ［J］. Food Chem ， 112 （ 2 ）： 388 - 393 .

NJOROGE S M ， UKEDA H ， SAWAMURA M ， 1996 . Changes in the volatile composition of Yuzu （ Citrus junos Tanaka） cold-pressed oil during storage ［J］. J Agric Food Chem ， 44 （ 2 ）： 550 - 556 .

RINCÓN A A ， PINO V ， AYALA J H ， et al ， 2014 . Multiple headspace solid-phase microextraction for quantifying volatile free fatty acids in cheeses ［J］. Talanta ， 129 ： 183 - 190 .

SAWAMURA M ， TU N T M ， ONISHI Y ， et al ， 2004 . Characteristic odor components of Citrus reticulata Blanco （Ponkan） cold-pressed oil ［J］. Biosci Biotechnol Biochem ， 68 （ 8 ）： 1690 - 1697 .

SERRANO E ， BELTRÁN J ， HERNÁNDEZ F ， 2009 . Application of multiple headspace-solid-phase microextraction followed by gas chromatography-mass spectrometry to quantitative analysis of tomato aroma components ［J］. J Chromatogr A ， 1216 （ 1 ）： 127 - 133 .

SHI Q ， GUO T ， YIN T ， et al ， 2018 . Classification of Pericarpium Citri Reticulatae of different ages by using a voltammetric electronic tongue system ［J］. Int J Electrochem Sci ， 13 （ 12 ）： 11359 - 11374 .

SU J ， WANG Y ， BAI M ， et al ， 2023 . Soil conditions and the plant microbiome boost the accumulation of monoterpenes in the fruit of Citrus reticulata ‘Chachi’ ［J］. Microbiome ， 11 （ 1 ）： 61 .

TAI Y N ， XU M ， REN J N ， et al ， 2016 . Optimisation of α-terpineol production by limonene biotransformation using Penicillium digitatum DSM 62840 ［J］. J Sci Food Agric ， 96 （ 3 ）： 954 - 961 .

TRANCHIDA P Q ， BONACCORSI I ， DUGO P ， et al ， 2012 . Analysis of Citrus essential oils： State of the art and future perspectives. A review ［J］. Flavour & Fragrance J ， 27 （ 2 ）： 98 - 123 .

WANG C ， ZHU M ， XIA W ， et al ， 2012 . Meta-analysis of Traditional Chinese Medicine in treating functional dyspepsia of liver-stomach disharmony syndrome ［J］. J Tradit Chin Med ， 32 （ 4 ）： 515 - 522 .

WANG H ， CHEN G ， FU X ， et al ， 2016 . Effects of aging on the phytochemical profile and antioxidative activity of Pericarpium Citri Reticulatae ‘Chachiensis’ ［J］. RSC Adv ， 6 （ 107 ）： 105272 - 105281 .

WEDLER H B ， PEMBERTON R P ， TANTILLO D J ， 2015 . Carbocations and the complex flavor and bouquet of wine： Mechanistic aspects of terpene biosynthesis in wine grapes ［J］. Molecules ， 20 （ 6 ）： 10781 - 10792 .

YI L ， DONG N ， LIU S ， et al ， 2015 . Chemical features of Pericarpium Citri Reticulatae and Pericarpium Citri Reticulatae Viride revealed by GC-MS metabolomics analysis ［J］. Food Chem ， 186 ： 192 - 199 .

YU L ， LI X ， LIU S ， et al ， 2009 . Comparative analysis of volatile constituents in Citrus reticulata Blanco using GC-MS and alternative moving window factor analysis ［J］. J Sep Sci ， 32 （ 20 ）： 3457 - 3465 .

YU X ， CHEN X ， LI Y ， et al ， 2022 . Effect of drying methods on volatile compounds of Citrus reticulata Ponkan and Chachi peels as characterized by GC-MS and GC-IMS ［J］. Foods ， 11 （ 17 ）： 2662 .

YUE F ， ZHANG F ， QU Q ， et al ， 2023 . Effects of ageing time on the properties of polysaccharide in tangerine peel and its bacterial community ［J］. Food Chem ， 417 ： 135812

ZHANG X ， GAO Z ， YANG Y ， et al ， 2022 . Rapid identification of the storage age of dried tangerine peel using a hand-held near infrared spectrometer and machine learning ［J］. J Infrared Spectrosc ， 30 （ 1 ）： 31 - 39 .

ZHENG Y ， ZENG X ， PENG W ， et al ， 2018 . Study on the discrimination between Citri Reticulatae Pericarpium Varieties based on HS-SPME-GC-MS combined with multivariate statistical analyses ［J］. Molecules ， 23 （ 5 ）： 1235 .

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SU Weiwei

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梅家齐

Related Institution

Department of Chemical Engineering， Maoming Polytechnic

Maoming Branch， Guangdong Laboratory for Lingnan Modern Agriculture

School of Life Sciences， Sun Yat-sen University / Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-valuation of Post-marketed TCM / Guangdong Key Laboratory of Plant Resources

．中山大学药学院生药学与天然药物化学实验室

 2．南方医科大学南方医院药学部

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Multiple headspace solid-phase microextraction for quantifying volatiles in Citrus reticulata 'Chachi' peel of different storage time

DOI：10.13471/j.cnki.acta.snus.ZR20240303

摘要

Abstract

关键词

Keywords

references