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Effects of extraction methods on cannabinoids in hemp leaves and
in vitro hypoglycemic and lipid-lowering functions- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 61, Issue 5, Pages: 94-104(2022)
- 作者机构：
1.东北林业大学林学院，黑龙江 哈尔滨 150040
2.黑龙江省森林食品资源利用重点实验室，黑龙江 哈尔滨 150040
- 作者简介：
- 基金信息：
DOI：10.13471/j.cnki.acta.snus.2021C021
CLC： TS209Published：25 September 2022，
Published Online：07 January 2022，
Received：22 September 2021，
Accepted：13 October 2021
扫 描 看 全 文
李德海,马可,张道明等.提取方法对汉麻叶大麻素成分及体外降糖降脂功能的影响[J].中山大学学报(自然科学版),2022,61(05):94-104.
LI Dehai,MA Ke,ZHANG Daoming,et al.Effects of extraction methods on cannabinoids in hemp leaves and in vitro hypoglycemic and lipid-lowering functions[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(05):94-104.
李德海,马可,张道明等.提取方法对汉麻叶大麻素成分及体外降糖降脂功能的影响[J].中山大学学报(自然科学版),2022,61(05):94-104. DOI： 10.13471/j.cnki.acta.snus.2021C021.
LI Dehai,MA Ke,ZHANG Daoming,et al.Effects of extraction methods on cannabinoids in hemp leaves and in vitro hypoglycemic and lipid-lowering functions[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2022,61(05):94-104. DOI： 10.13471/j.cnki.acta.snus.2021C021.
摘要
以汉麻浸膏得率、大麻二酚（CBD，cannabidiol）、四氢大麻酚（THC，tetrahydrocannabinol）含量为评价指标，研究了溶剂提取法、酶辅助提取法、超声波辅助与高剪切辅助对小叶汉麻中大麻素类成分的制备效果，进一步采用超高效液相高分辨质谱联用仪分析了大麻素成分，并对比分析了以上4种提取方法制备的小叶汉麻中大麻素浸膏的体外降糖降脂功能的差异性。试验结果表明：超声波辅助提取法制备的小叶汉麻大麻素效果最好，汉麻浸膏得率为7.06%，浸膏中
w
CBD
为283.04 mg/g，浸膏中THC未检出。超高效液相高分辨质谱联用仪对4种方法制备的汉麻浸膏进行检测，其中超声波辅助提取法含有的大麻素成分为8种，并且浸膏中
w
CBD
最高。体外降糖降脂试验结果表明，随着大麻素浸膏质量浓度的增加，4种提取方法制备大麻素的降糖降脂效果均显著提高，综合分析试验结果可以看出，超声波辅助提取法制备的大麻素浸膏降糖降脂效果最好，其中对
α
-葡萄糖苷酶抑制率的IC
50
值为0.20 mg/mL，
α
-淀粉酶的抑制率IC
50
值为0.50 mg/mL，对胆酸钠结合率的IC
50
值为4.88 mg/mL，甘氨胆酸钠结合率的IC
50
值为8.64 mg/mL，牛磺胆酸钠结合率的IC
50
值为12.14 mg/mL。本实验研究结果为汉麻叶大麻素成分制备及在辅助调节血糖血脂等健康产品中的应用提供参考。
Abstract
The extraction yield of hemp extract， cannabidiol （CBD） and tetrahydrocannabinol （THC） contents were used as evaluation indexes to investigate the effects of solvent extraction， enzyme assisted extraction， ultrasonic assisted and high shear assisted on the preparation of cannabinoids and the composition of cannabinoid was also analyzed by ultra-performance liquid mass spectrometry（UPLC）. The difference of the hypoglycemic and lipid-lowering functions of cannabinoid extract from hemp prepared by the above four extraction methods was further compared and analyzed
in vitro
. The results showed that the ultrasonic assisted extraction method had the best effect of cannabinoid， and the yield of hemp extract was 7.06%，
w
CBD
in the extract was 283.04 mg/g. THC was not detected in the extract. UPLC was used to detect the hemp extract prepared by four methods. Among them， ultrasound-assisted method contained 8 kinds of cannabinoids， and
w
CBD
in the extract was the highest. Hypoglycemic lipid-lowering
in vitro
test results showed that， with the increase of cannabinoid extract concentration， four kinds of extraction methods of preparing cannabinoid hypoglycemic lipid-lowering effect were significantly improved. Comprehensive analysis of test results showed that ultrasonic assisted preparation of cannabinoid extract hypoglycemic lipid-lowering effect is best， among which， alpha glycosidase enzyme inhibition rate of IC
50
value concentration was 0.20 mg/mL， the IC
50
value of α-amylase was 0.50 mg/mL， the IC
50
value of sodium cholate binding rate was 4.88 mg/mL， the IC
50
value of sodium glycocholate binding rate was 8.64 mg/mL， and the IC
50
value of sodium taurocholate binding rate was 12.14 mg/mL. The results of this study would provide references for the preparation of hemp leaf cannabinoid and its application in regulating blood glucose and lipid.
关键词
提取方法大麻素降糖降脂成分分析
Keywords
extraction methodscannabinoidlowering glucose and lipidcomposition analysis
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