1.广州中医药大学中药学院, 广东 广州 510006
2.华南农业大学资源环境学院, 广东 广州 510642
3.华南农业大学生命科学学院, 广东 广州 510642
4.丰城市人民医院药学部, 江西 丰城 331100
5.阳春市农业试验场(阳春市春砂仁试验场), 广东 阳春 529600
6.广东一方制药有限公司 / 广东省中药配方颗粒企业重点实验室, 广东 佛山 528244
7.广州达安基因股份有限公司, 广东 广州 510665
LÜ Bingding(20232110068@stu.gzucm.edu.cn)
ZHANG Yuyi(2454567366@qq.com);
TANG Liyun(tly1231@scau.edu.cn);
HU Jiajia(1015850392@qq.com);
SU Jing(573553847@qq.com);
XU Jie(935394848@qq.com);
HE Zhuohang(775900345@qq.com)
HE Guozhen (heguozhen@gzucm.edu.cn)
收稿:2025-10-20,
修回:2025-11-17,
录用:2025-11-18,
网络出版:2025-12,
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吕秉鼎, 张玉仪, 汤丽云, 等. 阳春砂果实发育过程中的表观性状变化与赤霉素相关基因的表达模式[J/OL]. 中山大学学报(自然科学版)(中英文), 2025,1-14.
LÜ Bingding, ZHANG Yuyi, TANG Liyun, et al. Morphological changes and gibberellin-related gene expression patterns during fruit growth and development of
吕秉鼎, 张玉仪, 汤丽云, 等. 阳春砂果实发育过程中的表观性状变化与赤霉素相关基因的表达模式[J/OL]. 中山大学学报(自然科学版)(中英文), 2025,1-14. DOI: 10.11714/acta.snus.ZR20250226.
LÜ Bingding, ZHANG Yuyi, TANG Liyun, et al. Morphological changes and gibberellin-related gene expression patterns during fruit growth and development of
阳春砂(
Amomum villosum
)的果实具有极高的药食两用价值,但其落果率高,产量低。为探究果实生长发育规律,本研究系统观测了阳春砂果实发育过程中的表观性状变化,并分析了赤霉素(GA,gibberellin)相关基因在果实及果柄发育过程中的表达模式。结果表明,阳春砂子房从授粉至成熟约需90 d,果实生长呈“慢-快-慢”趋势,鲜质量积累高峰出现在人工授粉后第14~25天。在授粉后第27天前,子房、果色、果皮厚度及果刺长度均发生显著变化。通过生物信息学分析,鉴定出10个赤霉素相关候选基因,包括1个
GA
13
ox
、1个
GA
20
ox
、1个
GA
3
ox
、2个
GA
2
ox
、4个
GID1c
和1个
DELLA
。果实与果柄中赤霉素相关基因的表达与果实生长发育及落果显著相关。研究结果系统解析了阳春砂果实生长发育规律及赤霉素相关基因的表达特性,为深入探究基因功能及筛选调控落果的关键候选基因提供了理论依据
The fruit of
Amomum villosum
is highly valued for its medicinal and edible properties. However
the fruit abscission rate is exceptionally high
resulting in low unit yield. To better understand the fruit growth and development
this study investigated the characteristic changes of fruits and the expression patterns of GA-related genes in both fruits and fruit stalks. Results revealed that it takes approximately 90 days for the ovary of
A. villosum
to reach maturity. Fruit growth pattern exhibited a slow-fast-slow trend
with the peak of fruit weight accumulation occurring between the 14 and 25 days after artificial pollination (DAP).
Before the 27 DAP
significant changes were observed in the ovary
fruit color
pericarp thickness
and fruit thorn length. Additionally
ten candidate GA-related genes
including 1
GA
13
ox
1
GA
20
ox
1
GA
3
ox
2
GA
2
oxs
4
GID1cs
and 1
DELLA
were analyzed using bioinformatics tools. The GA-related genes in fruit and fruit stalk exhibited significant correlations with fruit growth/development and fruit dropping. Overall
the fruit growth and development law
and the expression patterns of GA-related genes provided a theoretical foundation for further functional study and the screening of candidate genes for potential applications in
A. villosum
.
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