Applications of Radioactive Label in Biotechnology 1. Preparation of Thallic Trichloride and Radioiodination of Nucleic Acids as Probe

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Applications of Radioactive Label in Biotechnology 1. Preparation of Thallic Trichloride and Radioiodination of Nucleic Acids as Probe

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- Applications of Radioactive Label in Biotechnology 1. Preparation of Thallic Trichloride and Radioiodination of Nucleic Acids as Probe
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 27, Issue 1, (1988)
- 作者机构：
  
  1. 中山大学生物学系
  2. 中山大学昆虫学研究所
  3. 中山大学生物工程中心
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1988，
  
  Published Online：25 January 1988，
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Applications of Radioactive Label in Biotechnology 1. Preparation of Thallic Trichloride and Radioiodination of Nucleic Acids as Probe. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 27(1).(1988)
DOI：

Applications of Radioactive Label in Biotechnology 1. Preparation of Thallic Trichloride and Radioiodination of Nucleic Acids as Probe. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 27(1).(1988) DOI：

摘要

Abstract

The method of preparation of thallic trichloride has been established by using thallic chloride and chlorine. In the present of the mild oxidant thallic trichloride

the iodide ion reacts with the unsaturated bond between C-5 and C-6 of pyrimidines in ssDNA or ssRNA

and its DNA specific activity reached 4-6×10~6 cpm/μg. The specificity of the labels has been identified with molocular hybridization.

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