Peroxidases from Vinca rosea tissue cultures have been separated by polyacrylamide gel electrophoresis and isoelectric focusing into 6-13 isozymes. The isozyme pattern of callus tissues and those of plants at different development stages have been compared. The callus was obviously responsive to exogenous hormones.The isozyme pattern of the calluses cultured with different exogenous hormones was significantly changed. The subcultural and original callus had basically similar isozyme patterns when they were cultured under the same hormones condition.

60

Co irradiation of callus with dose of 25210 rad did not inhibit cellular growth and produced a new isozyme band.At different developmental stages of the plants

the isozyme patterns of their roots

stems and leaves were not similar

the healthy seedling’s have changed much than the young seedling’s and those of the old seedlings has three new isozyme bands at pI8-9.5