The method of carbon agglutintion test (CAT) was used to investigate the relatoin of the wolf spider and its prey

rice leafroller

Cnephalocrocis medinalis and was fund that the rate of positive reaction of CAT was 91.49% while the rate positive reaction of double diffusion test was 70.21% in 47 samples

the sensitivity of CAT has increased 128 times than positive reaction of double diffusion test

and cut short test time from 24-48 hrs to 1 hr.The method included preparation of antigen of prey

preparation of antiserum

purification of antibody

preparation of carbon antiserum

collection and preparation of predator and carbon agglutiotion test.