Studies on the Pathology of the Grass CarpⅢ.The Change of the relative activity of Malate Dehydrogenase(MDH)Isozyme in some Tissues and Organs of the Hemorrhagic Grass Carp

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Studies on the Pathology of the Grass CarpⅢ.The Change of the relative activity of Malate Dehydrogenase(MDH)Isozyme in some Tissues and Organs of the Hemorrhagic Grass Carp

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- Studies on the Pathology of the Grass CarpⅢ.The Change of the relative activity of Malate Dehydrogenase(MDH)Isozyme in some Tissues and Organs of the Hemorrhagic Grass Carp
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 34, Issue 1, Pages: 76-81(1995)
- 作者机构：
  
  1. 中山大学生物学系
  2. 华中农业大学水产学院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1995，
  
  Published Online：25 January 1995，
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Studies on the Pathology of the Grass CarpⅢ.The Change of the relative activity of Malate Dehydrogenase(MDH)Isozyme in some Tissues and Organs of the Hemorrhagic Grass Carp. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 34(1):76-81(1995)
DOI：

Studies on the Pathology of the Grass CarpⅢ.The Change of the relative activity of Malate Dehydrogenase(MDH)Isozyme in some Tissues and Organs of the Hemorrhagic Grass Carp. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 34(1):76-81(1995) DOI：

摘要

采用聚丙烯酰胺凝胶圆盘电泳方法，研究了正常草鱼和患出血病草鱼的肾脏、肝脏、脾脏、心脏、鳃、肌肉以及血清中的苹果酸脱氢酶（ＭＤＨ）同工酶。结果表明，患病草鱼和正常草鱼各组织器官及血清中的ＭＤＨ同工酶酶谱均为６条带，患病鱼的酶谱明显地不同于正常鱼的酶谱；ＭＤＨ同工酶的活性具有组织和器官的特异性，同一组织及器官，患病草鱼ＭＤＨ同工酶的总活性较正常草鱼偏高，其中鳃、肝脏、脾脏以及血清中的ＭＤＨ同工酶总活性具有显著性差异（ｐ＜０．０５）；同种组织及器官，患病草鱼的ＭＤＨ同工酶的相应组分，活性序列产生了较大的差异；ＭＤＨ同工酶可以作为草鱼患出血病的一个生理、病理指标。

Abstract

By using polyacrylamide gel e lectrophoresis

the changes in the relati ve activity ofmalate dehydrogenase(MDH)isozymes in the gill

liver

blood serum

heart

spleen

muscleand kidney of hemorrhagic grass carp were studied.It was shown all these tissues and organsand blood serum possess six kind of ribbons and the electrophoretogram of MDH isozymes in the healthy and diseased fish was different. The MDH isozymes activity apparently exhibited a tissue-specific expression. The total activity of the MDH isozyme of the diseased fish was considerably higher than that of the healthy in various tissues and organs.the activity of the isozyme s in the gill

liver

spleen and blood serum showed a conspicuous differently(p<0. 05).and theactivity order of the MDH isozymes showed more differently in the same tissue and drgan

Basedon the above results

it is considered that MDH isozyme electrophore togram of the tissues andorgans can be used as a physiological as well as pathological index of the hemorrhagic disease of grass carp.

关键词

&nbsp草鱼出血病同工酶

Keywords

&nbspgrass carphemorrhagic diseaseisozyme

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