The fluorescent leakage from Brassicu alboglabra Bailey

B. chinensis Linn

and B.parachinensis bailey was identified by paper chromatography.The leakage was found to contain acampound

the R_f value and color reaction of which were similar to that of sinapine

The concen-tration of the sinapine determinated by using the specific absorbance of the compound at 388nmin basic solution(pH> 1 0)was closely associated with the seed vigor.Their relative coefficiencywere respectively-0. 92

-0. 81 and -0. 81.Thus

the seed vigor test in these species utilizingsinapine determination is practical

sensitive and better than method of observation of the fluores-cent leakage under UV light.