Two primers containing the Pst I site and Sal I site and stop codon respectively were synthesized and used to amplify a 232 hp DNA sequence coding for mature hIGF-1 protein with the 700 bp hIGF-1 cDNA fragment as template by Taq polymerase.The amplified DNA fragment was cloned into M13mp18 vector and sequenced by Sanger-s dideoxy chain termination method. The sequencing data show that the nucleotide sequence of the cloned fragment is the same as hIGF-1 coding sequence published and that we have successfully remoulded the hIGF-1 protein coding region with Pst I site at 5’ end and Sal I site and stop codon at 3’ end using the add-on PCR technique.