This paper dealed with mass individual rearing the larvae of the cotton leafworm

Spodptera litura F

on an artificial diet for propagating S

litura nuclear polyhedrosis virus(SINPV).The use of different combinations of six virus concentrations with three inoculating in-stars

six rearing temperatures with two inoculating instars and an artificial diet with a natural di-et

to sereen an optimal condition

The results showed that the maximal yield of SINPV wasmade when the larvae were treated with 3.85×10￣5 PIBs/mL in 3rd instar and3.85×10￣7 PIBs/mL in 4th instar.The optimal temperature was 2 4～27℃ when the larvae were infeeted in 4thinstar

nd the yield of the SINPV was higher rearing the larvae in an artificial diet than in a natural diet. here was a linear correlation between the cadver weights and the SINPV yields respectively (P<0. 01).The mortality rate of the infected larvae was directly proportional to temperature。