Acta Scientiarum Naturalium Universitatis SunYatseniVol. 35, Issue S2, Pages: 54-57(1996)
作者机构:
中山大学生命科学学院
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Published:1996,
Published Online:25 December 1996,
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Wang Yiliang, Zhang Tianyuan, Luo Jinxian. Selection of Pullulanase Producing Strain A 3345. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 35(S2):54-57(1996)
DOI:
Wang Yiliang, Zhang Tianyuan, Luo Jinxian. Selection of Pullulanase Producing Strain A 3345. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 35(S2):54-57(1996)DOI:
A Aerobacter aerogenes mutant producing high pullulanas eactivity was selected by reciprocal or mixed treatment of the parental strain with NTG; U.V and HNO 2. The pullulanase activity of the selected strain is 750 u/mL in Teng Wei (D M) medium (A) after 4 days incobation at 30℃
which is 110% higher than the parental strain. The optimal pH and temperature is 5 2~6 4 and 50℃ respectively.