Molecular Cloning and Expression Analysis of Fibrinogen βGene from Japanese Flounder (Paralichthys olivaceus)

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Molecular Cloning and Expression Analysis of Fibrinogen βGene from Japanese Flounder (Paralichthys olivaceus)

更新时间：2023-12-11

- Molecular Cloning and Expression Analysis of Fibrinogen βGene from Japanese Flounder (Paralichthys olivaceus)
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 48, Issue 6, Pages: 89-94(2009)
- 作者机构：
  
  1. 中国海洋大学海洋生物遗传学与基因资源利用教育部重点实验室,山东,青岛,266003
  2. 
  3. 长江水产研究所,湖北,荆州,434000
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2009，
  
  Published Online：25 November 2009，
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Molecular Cloning and Expression Analysis of Fibrinogen βGene from Japanese Flounder (Paralichthys olivaceus). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 48(6):89-94(2009)
DOI：

Molecular Cloning and Expression Analysis of Fibrinogen βGene from Japanese Flounder (Paralichthys olivaceus). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 48(6):89-94(2009) DOI：

摘要

通过mRNA差异显示发现一个鳗弧菌刺激后在牙鲆肝脏中表达量显著增加的片段，结合RACE技术得到了该差异片段1 856 bp的全长cDNA，包含一个1 479 bp的开放阅读框，编码的蛋白质含有fibrinogen β的C末端签名序列。同源性分析表明其氨基酸序列与鱼类以及哺乳类的fibrinogen β都具有高度的相似性，因此可断定此基因为牙鲆的fibrinogen β基因(GenBank登录号为EF581895)。RT-PCR分析表明，在注射鳗弧菌后，该基因在肝脏、肾脏、脾脏、腮、肠、心脏中的表达量呈现逐渐增加的趋势。推测其可能在鳗弧菌侵染中，起到了促进伤口愈合的作用，或通过和其他细胞因子结合在防御细菌的侵染中发挥免疫调节作用。

Abstract

Key words:

Paralichthys olivaceus

fibrinogen β

cloning

expression

关键词

牙鲆 Paralichthys olivaceusfibrinogen β克隆组织表达

Keywords

Paralichthys olivaceusfibrinogen βcloningexpression

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Related Institution

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Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yatsen University

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