To establish fingerprints of  Tripterygium preparations from different manufacturers，and to simultaneously determine the contents of four active components by ultra performance liquid chromatography (UPLC)，providing a rapid detection method for quality control of  Tripterygium preparations. UPLC separation was performed on a BEH Shield RP 18 column (2.1 mm×100 mm，1.7 μm) with a mobile phase of acetonitrile-water under gradient elution，the flow rate was 0.25 mL/min and the column temperature was 35 ℃，the detection wavelength was set at 220 nm. The similarity of UPLC fingerprints was evaluated by qualitative and quantitative analysis，respectively. The results showed that the fingerprints of  Tripterygium preparations from different manufacturers were found to be in great diverse，while the contents of four active components were significantly different. So

this method is specific，fast，and with good resolution for the determination of UPLC fingerprint of  Tripterygium preparations. The simultaneous determination of contents of four active components can be used for the quality control of  Tripterygium preparations to ensure the safety and utility in clinical medication.