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华南农业大学海洋学院,广东 广州 510642
Received:13 February 2026,
Revised:2026-04-17,
Accepted:20 April 2026,
Online First:2026-05,
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Huang Cuifen, Lan Yinmei, Yang Wenfeng, et al. Effects of
Huang Cuifen, Lan Yinmei, Yang Wenfeng, et al. Effects of
哈维氏弧菌(
Vibrio harveyi
)是海水经济鱼类的重要病原,给我国养殖业造成了巨大的经济损失。本研究以斜带石斑鱼(
Epinephelus coioides
)为对象,解析哈维氏弧菌感染石斑鱼后引起的肠道菌群结构及免疫反应的变化。结果表明,相较于对照组,哈维氏弧菌感染的石斑鱼肠道菌群结构发生了明显的变化:变形菌门(Proteobacteria)的相对丰度从79.06%降至56.22%,厚壁菌门(Firmicutes)从5.46%升至12.90%,拟杆菌门(Bacteroidetes)从2.78%升至9.80%,放线菌门(Actinobacteria)从1.97%升至2.47%,潜在致病菌[如假交替单胞菌属(
Pseudoalteromonas
)]相对丰度出现或增加,而潜在有益菌[如嗜冷杆菌属(
Psychrobacter
)]比例下降。哈维氏弧菌感染后,石斑鱼差异表达显著的基因主要在溶菌酶活性、肽聚糖水解活性、氧化还原酶活性及谷胱甘肽代谢等多条免疫、抗氧化及代谢相关的通路中。研究结果揭示病原菌可直接影响宿主肠道菌群结构、免疫关键基因的表达,因此,有效维护鱼类肠道健康对防控病害造成的损伤程度具有重要意义。
As a significant pathogen of marine fish,
Vibrio harveyi
has caused huge economic losses of marine economic fish farming industry in China. In this study, we investigated the changes of intestinal microbial community structure and immune responses in
Epinephelus coioides
during
V. harveyi
infection. Results showed that
V. harveyi
infection altered the intestinal microbial community structure: the relative abundance of Proteobacteria decreased from 79.06% to 56.22%, while Firmicutes increased from 5.46% to 12.90%, Bacteroidetes changed from 2.78% to 9.80% and Actinobacteria changed from 1.97% to 2.47%, indicating that the emergence or increased relative abundance of potential pathogens such as
Pseudoalteromonas
, and decreased proportions of beneficial bacteria such as
Psychrobacter
. Meanwhile, differentially expressed genes enriched in GO terms and metabolic pathways related to immunity and antioxidant defense, including lysozyme activity, peptidoglycan muralytic activity, oxidoreductase activity and glutathione metabolism. These findings reveal that pathogenic bacteria can directly influence the structure of intestinal microbial community structure, as well as the extent of changes in host immune organs and key immune genes. Therefore, it is important to protect the intestinal health of farmed f
ish for disease prevention.
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