中山大学药学院,广东 广州 510006
崔清雅(2001年生),女;研究方向:天然药物化学;E-mail:cuiqy5@mail2.sysu.edu.cn
唐贵华(1983年生),男;研究方向:天然药物化学;E-mail:tanggh5@mail.sysu.edu.cn
收稿:2026-05-12,
修回:2026-06-11,
录用:2026-06-22,
网络首发:2026-09,
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崔清雅, 招展涛, 李磊, 等. 两株天麻内生曲霉共培养的次生代谢产物[J/OL]. 中山大学学报(自然科学版)(中英文), 2026,1-6.
Cui Qingya, Zhao Zhantao, Li Lei, et al. Secondary metabolites from co-culture of two endophytic
崔清雅, 招展涛, 李磊, 等. 两株天麻内生曲霉共培养的次生代谢产物[J/OL]. 中山大学学报(自然科学版)(中英文), 2026,1-6. DOI: 10.11714/acta.snus.ZR20260127.
Cui Qingya, Zhao Zhantao, Li Lei, et al. Secondary metabolites from co-culture of two endophytic
采用大米培养基对两株天麻内生曲霉
Aspergillus
sp. TMw与
A. flavus
TM1进行共培养,利用层析技术对发酵产物进行分离纯化,通过现代波谱学技术进行结构鉴定。从中分离得到11个已知化合物,分别为ditryptophenaline(
1
)、cyclo-(
N
-methylphenylalanyltryptophenyl)(
2
)、1,3,9-trimethyluric acid(
3
)、曲酸(
4
)、methyl 5-(hydroxymethyl)furan-3-carboxylate(
5
)、β-谷甾酮(
6
)、phomaligol A(
7
)、phomapyrone C(
8
)、双酚A (
9
)、对羟基苯甲醛(
10
)和4-(2-乙氧基乙基)苯酚(
11
)。对化合物
1
~
11
采用CCK-8法进行细胞毒活性测试,结果显示化合物
1
对非小细胞肺癌细胞H1975具有细胞毒性,IC
50
值为(11.69±1.56) μmol/L,其余化合物的活性较弱或无细胞毒活性。
The co-culture of
Aspergillus
sp. TMw and
A. flavus
TM
1, two endophytic fungi isolated from
Gastrodia elata
,was carried out using rice medium. The fermentation products were isolated and purified by chromatographic techniques,and their structures were identified by modern spectroscopic methods. A total of 11 known compounds were obtained,namely ditryptophenaline(
1
),cyclo-(
N
-methylphenylalanyltryptophenyl)(
2
),1,3,9-trimethyluric acid(
3
),kojic acid(
4
),methyl 5-(hydroxymethyl)furan-3-carboxylate(
5
),β-sitostenone(
6
),phomaligol A(
7
),phomapyrone C(
8
),bisphenol A(
9
),4-hydroxybenzaldehyde(
10
),and 4-(2-ethoxyethyl)phenol(
11
). The cytotoxic activities of compounds
1
-
11
were evaluated using the CCK-8 method. The results showed that only compound
1
exhibited cytotoxicity against non-small cell lung cancer cell line H1975 with an IC
50
value of (11.69±1.56) μmol/L
while other compounds exhibited weaker or no cytotoxic activity.
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