Effects of polycyclic aromatic hydrocarbons on the photosynthesis system of<italic style="font-style: italic"> Raphidocelis subcapitata</italic> and transcriptional regulatory mechanisms

Li Yue; Luo Lijuan; Luan Tiangang

doi:10.11714/acta.snus.ZR20260052

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Effects of polycyclic aromatic hydrocarbons on the photosynthesis system of Raphidocelis subcapitata and transcriptional regulatory mechanisms

更新时间：2026-05-25

- Effects of polycyclic aromatic hydrocarbons on the photosynthesis system of Raphidocelis subcapitata and transcriptional regulatory mechanisms
- Acta Scientiarum Naturalium Universitatis Sunyatseni Pages: 1-10(2026)
- 作者机构：
  
  1.广东省流域水环境治理与水生态修复重点实验室 / 广东工业大学生态环境与海洋学院，广东广州510006
  2.化学与精细化工广东省实验室揭阳分中心，广东揭阳 515200
  3.水产动物疫病防控与健康养殖全国重点实验室 / 中山大学生命科学学院，广东广州 510275
- 作者简介：
- 基金信息：
- DOI：10.11714/acta.snus.ZR20260052
  CLC： X17
- Received：02 March 2026，
  
  Revised：2026-04-15，
  
  Accepted：20 April 2026，
  
  Online First：2026-05，
- 稿件说明：
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Li Yue, Luo Lijuan, Luan Tiangang. Effects of polycyclic aromatic hydrocarbons on the photosynthesis system of Raphidocelis subcapitata and transcriptional regulatory mechanisms[J/OL]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2026, 1-10.
DOI：

Li Yue, Luo Lijuan, Luan Tiangang. Effects of polycyclic aromatic hydrocarbons on the photosynthesis system of Raphidocelis subcapitata and transcriptional regulatory mechanisms[J/OL]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2026, 1-10. DOI： 10.11714/acta.snus.ZR20260052.

摘要

多环芳烃（PAHs，polycyclic aromatic hydrocarbons）是一类广泛分布于大气、水体和土壤中的持久性有机污染物，具有致畸性、致癌性和致突变性，严重威胁水生态系统安全。藻类作为水生生态系统的初级生产者，其光合系统是PAHs毒理作用的敏感靶点，但目前PAHs对藻类光合系统的损伤及转录调控机制尚未明确。本研究以模式淡水绿藻——近头状尖胞藻（

Raphidocelis subcapitata

）为研究对象，结合叶绿素荧光成像技术与转录组学分析方法，探究PAHs对藻类光合系统的影响及其调控机制。研究结果表明，苯并［a］芘对近头状尖胞藻最大光化学效率的抑制作用整体弱于菲（PHE）和芘（PYR），PHE和PYR处理组藻的电子传递速率分别降至对照组的43%和49%，而PAHs对藻的非光化学猝灭系数影响较小。藻类光合系统的5个关键基因（

cabII-1

、

LHCA4

、

LHCA5

、

PsbP

和

PsaN

）表达呈时间依赖性，在暴露第3~5天呈显著上调趋势，至第7天表达量回落；随着暴露时间的延长，藻类光合系统的转录调控机制逐渐失衡，最终导致光合功能持续受损。本实验从生理与分子水平明确了PAHs对藻类光合系统的损伤机制及转录调控特征，为PAHs的生态毒理及水生生态风险评估提供理论参考。

Abstract

Polycyclic aromatic hydrocarbons （PAHs） are a class of persistent organic pollutants widely distributed in the air， water and soil. They are teratogenic， carcinogenic and mutagenic， posing a serious threat to the safety of aquatic ecosystems. As primary producers in aquatic ecosystems， algal photosynthetic systems are sensitive targets of PAHs toxicity， but the mechanisms of PAHs-induced damage and transcriptional regulation on algal photosynthetic systems remain unclear. In this study， the model freshwater green alga

Raphidocelis subcapitata

was used as the research object， combining chlorophyll fluorescence imaging technology and transcriptomic analysis to explore the effects of PAHs on the algal photosynthetic system and its regulatory mechanism. The results showed that the inhibitory effect of benzo［a］pyrene on the maximum photochemical efficiency of

R. subcapitata

was generally weaker than that of phenanthrene （PHE） and pyrene （PYR）. The electron transport rate of algae in the PHE and PYR treatment groups decreased to 43% and 49% of the control group， respectively， while PAHs exposure had little effect on the non-photochemical quenching coefficient of algae. The expression of five key genes in the algal photosynthetic system （

cabII-1

，

LHCA4

，

LHCA5

，

PsbP

and

PsAN

） was time-dependent， showing a significant upward trend on days 3 to 5 of exposure and decreasing on day 7. With the extension of exposure time， the transcriptional regulation mechanism of the algal photosynthetic system gradually became imbalanced， eventually leading to continuous damage to photosynthetic function. This study clarified the damage mechanism

and transcriptional regulation characteristics of PAHs on the algal photosynthetic system at the physiological and molecular levels， providing a theoretical reference for the ecotoxicological study of PAHs and the assessment of aquatic ecological risks.

关键词

Keywords

references

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Effects of polycyclic aromatic hydrocarbons on the photosynthesis system of Raphidocelis subcapitata and transcriptional regulatory mechanisms

DOI：10.11714/acta.snus.ZR20260052

摘要

Abstract

关键词

Keywords

references